Effects of lipoic acid and n-3 long-chain polyunsaturated fatty acid on the liver ovariectomized rat model of menopause.

BACKGROUND: Bilateral ovariectomy is an experimental model used to analyse the effects of menopause and develop strategies to mitigate the deleterious effects of this condition. Supplementation of the diet with antioxidants has been used to reduce potential oxidative stress caused by menopause. The purpose of the study was to analyse the effects of α-lipoic acid (LA), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), dietary supplementation on oxidative stress in the livers of ovariectomized rats. METHODS: In this study, we evaluated the effect of dietary supplementation with LA, DHA and EPA for a period of 16 weeks on oestrogen levels and oxidative stress biomarkers in the livers of ovariectomized 25 three-month-old rats. RESULTS: Serum oestrogen levels were lower after ovariectomy but were not altered by dietary treatments. LA was capable of acting in the liver, recovering the activity of the antioxidant enzymes superoxide dismutase and glutathione peroxidase, and reducing protein oxidative damage. Moreover, LA supplementation reduced nitrite and nitrate levels. DHA and EPA recovered the antioxidant activity of cytosolic and mitochondrial superoxide dismutase, decreasing protein oxidation. Protection against lipid oxidation differed between treatments. The DHA-treated group showed increased levels of the lipid peroxidation biomarker malondialdehyde compared to the ovariectomized group. However, malondialdehyde levels were not altered by EPA treatment. CONCLUSIONS: The results suggest that the antioxidant response varies among evaluated supplementations and all supplements were able to alter enzymatic and non-enzymatic antioxidants in the livers of ovariectomized rats. DHA presented the most evident antioxidant effect, decreasing protein and lipid damage.

Redox changes in the brains of reproductive female rats during aging.

Reproduction is a critical and demanding phase of an animal's life. In mammals, females usually invest much more in parental care than males, and lactation is the most energetically demanding period of a female's life. Here, we tested whether oxidative stress is a consequence of reproduction in the brains of female Wistar rats. We evaluated the activities of glutathione peroxidase, glutathione S-transferase, and superoxide dismutase; H2O2 consumption; protein carbonylation; NO2 & NO3 levels; and total glutathione, as well as sex hormone levels in brain tissue of animals at 3, 6, 12, and 24months of age. Animals were grouped according to reproductive experience: breeders or non-breeders. Most of the studied parameters showed a difference between non-breeders and breeders at 12 and 24months. At 24months of age, breeders showed higher superoxide dismutase activity, H2O2 consumption, glutathione peroxidase activity, and carbonyl levels than non-breeders. In 12-month-old non-breeders, we observed a higher level of H2O2 consumption and higher superoxide dismutase and glutathione peroxidase activities than breeders. By evaluating the correlation network, we found that there were a larger number of influential nodes and positive links in breeder animals than in non-breeders, indicating a greater number of redox changes in breeder animals. Here, we also demonstrated that the aging process caused higher oxidative damage and higher antioxidant defenses in the brains of breeder female rats at 24months, suggesting that the reproduction process is costly, at least for the female brain. This study shows that there is a strong potential for a link between the cost of reproduction and oxidative stress.